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plasmid encoding egfp cre recombinase  (Addgene inc)


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    Addgene inc plasmid encoding egfp cre recombinase
    Plasmid Encoding Egfp Cre Recombinase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+encoding+egfp+cre+recombinase/CAG-GFP-IRES-CRE+(Plasmid+%2348201)/pmc11584804-314-9-20
    Average 93 stars, based on 21 article reviews
    plasmid encoding egfp cre recombinase - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Dynamics of endogenous PARP1 and PARP2 during DNA damage revealed by live-cell single-molecule imaging
    Article Snippet: .. These cells (1.5 × 10 6 ) were transfected with 2 μg of plasmid encoding eGFP-Cre recombinase (Addgene # 11923), a gift from Brian Sauer ( ). ..

    Article Title: Single-molecule imaging reveals the kinetics of non-homologous end-joining in living cells
    Article Snippet: .. These cells were then transfected with 2 μg of plasmid encoding eGFP-Cre recombinase (CAG-GFP-IRES-CRE was a gift from Fred Gage, Addgene plasmid # 48201) . ..

    Article Title: Dynamics of endogenous PARP1 and PARP2 during DNA damage revealed by live-cell single-molecule imaging.
    Article Snippet: .. These cells (1.5 3 106) were transfected with 2 mg of plasmid encoding eGFP-Cre recombinase (Addgene # 11923), a gift from Brian Sauer.85 To obtain individual clones, cells expressing eGFP were subjected to sorting into single wells of multiple 96-well cell culture plates. .. Upon expansion, DNA from these cells was extracted using QuickExtract DNA extraction solution (Lucigen # QE09050) and used as a template for confirmation of homologous recombination by PCR and Sanger sequencing.

    Article Title: Single-molecule imaging reveals the kinetics of non-homologous end-joining in living cells
    Article Snippet: .. These cells were transfected with 2 μg of plasmid encoding eGFP-Cre recombinase (CAG-GFP-IRES-CRE was a gift from Fred Gage, Addgene plasmid Nr. 48201) . ..

    Article Title: Dynamics of endogenous PARP1 and PARP2 during DNA damage revealed by live-cell single-molecule imaging
    Article Snippet: .. These cells (1.5 × 10 6 ) were transfected with 2 μg of plasmid encoding eGFP-Cre recombinase (Addgene # 11923), a gift from Brian Sauer. ..

    Article Title: Single-molecule imaging reveals the kinetics of non-homologous end-joining in living cells.
    Article Snippet: .. These cells were then transfected with 2 μg of plasmid encoding eGFP-Cre recombinase (CAG-GFP-IRES-CRE was a gift from Fred Gage, Addgene plasmid # 48201)55. ..

    Plasmid Preparation:

    Article Title: Dynamics of endogenous PARP1 and PARP2 during DNA damage revealed by live-cell single-molecule imaging
    Article Snippet: .. These cells (1.5 × 10 6 ) were transfected with 2 μg of plasmid encoding eGFP-Cre recombinase (Addgene # 11923), a gift from Brian Sauer ( ). ..

    Article Title: Single-molecule imaging reveals the kinetics of non-homologous end-joining in living cells
    Article Snippet: .. These cells were then transfected with 2 μg of plasmid encoding eGFP-Cre recombinase (CAG-GFP-IRES-CRE was a gift from Fred Gage, Addgene plasmid # 48201) . ..

    Article Title: Dynamics of endogenous PARP1 and PARP2 during DNA damage revealed by live-cell single-molecule imaging.
    Article Snippet: .. These cells (1.5 3 106) were transfected with 2 mg of plasmid encoding eGFP-Cre recombinase (Addgene # 11923), a gift from Brian Sauer.85 To obtain individual clones, cells expressing eGFP were subjected to sorting into single wells of multiple 96-well cell culture plates. .. Upon expansion, DNA from these cells was extracted using QuickExtract DNA extraction solution (Lucigen # QE09050) and used as a template for confirmation of homologous recombination by PCR and Sanger sequencing.

    Article Title: Single-molecule imaging reveals the kinetics of non-homologous end-joining in living cells
    Article Snippet: .. These cells were transfected with 2 μg of plasmid encoding eGFP-Cre recombinase (CAG-GFP-IRES-CRE was a gift from Fred Gage, Addgene plasmid Nr. 48201) . ..

    Article Title: Dynamics of endogenous PARP1 and PARP2 during DNA damage revealed by live-cell single-molecule imaging
    Article Snippet: .. These cells (1.5 × 10 6 ) were transfected with 2 μg of plasmid encoding eGFP-Cre recombinase (Addgene # 11923), a gift from Brian Sauer. ..

    Article Title: Single-molecule imaging reveals the kinetics of non-homologous end-joining in living cells.
    Article Snippet: .. These cells were then transfected with 2 μg of plasmid encoding eGFP-Cre recombinase (CAG-GFP-IRES-CRE was a gift from Fred Gage, Addgene plasmid # 48201)55. ..

    Clone Assay:

    Article Title: Dynamics of endogenous PARP1 and PARP2 during DNA damage revealed by live-cell single-molecule imaging.
    Article Snippet: .. These cells (1.5 3 106) were transfected with 2 mg of plasmid encoding eGFP-Cre recombinase (Addgene # 11923), a gift from Brian Sauer.85 To obtain individual clones, cells expressing eGFP were subjected to sorting into single wells of multiple 96-well cell culture plates. .. Upon expansion, DNA from these cells was extracted using QuickExtract DNA extraction solution (Lucigen # QE09050) and used as a template for confirmation of homologous recombination by PCR and Sanger sequencing.

    Expressing:

    Article Title: Dynamics of endogenous PARP1 and PARP2 during DNA damage revealed by live-cell single-molecule imaging.
    Article Snippet: .. These cells (1.5 3 106) were transfected with 2 mg of plasmid encoding eGFP-Cre recombinase (Addgene # 11923), a gift from Brian Sauer.85 To obtain individual clones, cells expressing eGFP were subjected to sorting into single wells of multiple 96-well cell culture plates. .. Upon expansion, DNA from these cells was extracted using QuickExtract DNA extraction solution (Lucigen # QE09050) and used as a template for confirmation of homologous recombination by PCR and Sanger sequencing.

    Cell Culture:

    Article Title: Dynamics of endogenous PARP1 and PARP2 during DNA damage revealed by live-cell single-molecule imaging.
    Article Snippet: .. These cells (1.5 3 106) were transfected with 2 mg of plasmid encoding eGFP-Cre recombinase (Addgene # 11923), a gift from Brian Sauer.85 To obtain individual clones, cells expressing eGFP were subjected to sorting into single wells of multiple 96-well cell culture plates. .. Upon expansion, DNA from these cells was extracted using QuickExtract DNA extraction solution (Lucigen # QE09050) and used as a template for confirmation of homologous recombination by PCR and Sanger sequencing.



    Similar Products

    plasmid encoding egfp cre recombinase  (Addgene inc)
    93
    Addgene inc plasmid encoding egfp cre recombinase
    Plasmid Encoding Egfp Cre Recombinase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+encoding+egfp+cre+recombinase/CAG-GFP-IRES-CRE+(Plasmid+%2348201)/pmc11584804-314-9-20
    Average 93 stars, based on 1 article reviews
    plasmid encoding egfp cre recombinase - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    retrograde aav encoding cre recombinase  (Addgene inc)
    96
    Addgene inc retrograde aav encoding cre recombinase
    Figure 5. Silencing the S2!nwS1 pathway impairs both rotarod performance and run- way locomotion. A, Experimental paradigm. <t>Retrograde</t> <t>AAV-Cre</t> was injected into nwS1. AAV encoding the Cre-dependent hM4Di-mCherry was injected into S2 or M1. Three weeks later, mice received an intraperitoneal injection of CNO (CNO-2HCl, 4.13 mg/kg) or the equiv- alent volume of saline 1 h before testing on the rotarod and the locomotion assay. B, C, Intersectional Cre-lox approach resulted in hM4Di-mCherry expression localized to S2 or M1. Demarcations are based on a mouse brain atlas (Paxinos and Franklin, 2012). D, Average rotarod performance of mice expressing hM4Di in S2!nwS1 with CNO or saline injection. Saline: 222 6 5.77 s, 7 mice; CNO: 183 6 5.45 s, 8 mice; p = 0.0003, t test. E, Average rotarod performance of mice expressing hM4Di in M1!nwS1 with CNO or saline injection. Saline: 214 6 19.1 s, 6 mice; CNO: 198 6 12.3 s, 6 mice; p = 0.52, t test. F, Rear view images of a mouse walking on the runway before and after S2!nwS1 inactivation with hM4Di. G, Mean right hind paw angles in mice before and after the S2!nwS1 or M1!nwS1 inactivation. Each data point represents a mean of the hind paw angles during multiple strides observed in a single mouse. S2!nwS1 inactivation; saline: 25.8 6 1.30°; CNO: 31.8 6 2.24°; 6 mice; p = 0.008, paired t test. M1!nwS1 inactivation; saline: 27.96 1.50°; CNO: 28.06 2.64°; 3 mice; p = 0.930, paired t test.
    Retrograde Aav Encoding Cre Recombinase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+encoding+egfp+cre+recombinase/pENN%2EAAV%2EhSyn%2EHI%2EeGFP-Cre%2EWPRE%2ESV40+(Plasmid+%23105540)/10__1523_slash_jneurosci__0994___21__2021-111-11-21
    Average 96 stars, based on 1 article reviews
    retrograde aav encoding cre recombinase - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 5. Silencing the S2!nwS1 pathway impairs both rotarod performance and run- way locomotion. A, Experimental paradigm. Retrograde AAV-Cre was injected into nwS1. AAV encoding the Cre-dependent hM4Di-mCherry was injected into S2 or M1. Three weeks later, mice received an intraperitoneal injection of CNO (CNO-2HCl, 4.13 mg/kg) or the equiv- alent volume of saline 1 h before testing on the rotarod and the locomotion assay. B, C, Intersectional Cre-lox approach resulted in hM4Di-mCherry expression localized to S2 or M1. Demarcations are based on a mouse brain atlas (Paxinos and Franklin, 2012). D, Average rotarod performance of mice expressing hM4Di in S2!nwS1 with CNO or saline injection. Saline: 222 6 5.77 s, 7 mice; CNO: 183 6 5.45 s, 8 mice; p = 0.0003, t test. E, Average rotarod performance of mice expressing hM4Di in M1!nwS1 with CNO or saline injection. Saline: 214 6 19.1 s, 6 mice; CNO: 198 6 12.3 s, 6 mice; p = 0.52, t test. F, Rear view images of a mouse walking on the runway before and after S2!nwS1 inactivation with hM4Di. G, Mean right hind paw angles in mice before and after the S2!nwS1 or M1!nwS1 inactivation. Each data point represents a mean of the hind paw angles during multiple strides observed in a single mouse. S2!nwS1 inactivation; saline: 25.8 6 1.30°; CNO: 31.8 6 2.24°; 6 mice; p = 0.008, paired t test. M1!nwS1 inactivation; saline: 27.96 1.50°; CNO: 28.06 2.64°; 3 mice; p = 0.930, paired t test.

    Journal: The Journal of Neuroscience

    Article Title: A Cortico-Cortical Pathway Targets Inhibitory Interneurons and Modulates Paw Movement during Locomotion in Mice

    doi: 10.1523/jneurosci.0994-21.2021

    Figure Lengend Snippet: Figure 5. Silencing the S2!nwS1 pathway impairs both rotarod performance and run- way locomotion. A, Experimental paradigm. Retrograde AAV-Cre was injected into nwS1. AAV encoding the Cre-dependent hM4Di-mCherry was injected into S2 or M1. Three weeks later, mice received an intraperitoneal injection of CNO (CNO-2HCl, 4.13 mg/kg) or the equiv- alent volume of saline 1 h before testing on the rotarod and the locomotion assay. B, C, Intersectional Cre-lox approach resulted in hM4Di-mCherry expression localized to S2 or M1. Demarcations are based on a mouse brain atlas (Paxinos and Franklin, 2012). D, Average rotarod performance of mice expressing hM4Di in S2!nwS1 with CNO or saline injection. Saline: 222 6 5.77 s, 7 mice; CNO: 183 6 5.45 s, 8 mice; p = 0.0003, t test. E, Average rotarod performance of mice expressing hM4Di in M1!nwS1 with CNO or saline injection. Saline: 214 6 19.1 s, 6 mice; CNO: 198 6 12.3 s, 6 mice; p = 0.52, t test. F, Rear view images of a mouse walking on the runway before and after S2!nwS1 inactivation with hM4Di. G, Mean right hind paw angles in mice before and after the S2!nwS1 or M1!nwS1 inactivation. Each data point represents a mean of the hind paw angles during multiple strides observed in a single mouse. S2!nwS1 inactivation; saline: 25.8 6 1.30°; CNO: 31.8 6 2.24°; 6 mice; p = 0.008, paired t test. M1!nwS1 inactivation; saline: 27.96 1.50°; CNO: 28.06 2.64°; 3 mice; p = 0.930, paired t test.

    Article Snippet: For pathway-specific inactivation, AAV9hSyn-DIO-hM4D(Gi)-mCherry (titer 2.5 1013 GC/ml, Addgene 44362) and retrograde AAV encoding Cre recombinase (retroAAVhSyn-HI-eGFP-Cre (titer 1.17 1013 GC/ml, Addgene 105540) were stereotaxically injected into S2 or M1 and nwS1, respectively (coordinates from bregma: S2, 1.2 mm posterior, 5.5 mm lateral; M1 1.0 mm anterior, 1.0 mm lateral).

    Techniques: Injection, Saline, Expressing